Author : Ashwin singh chouhan
Keyword : Antioxidant, hepatoprotective, ddph, holarrhena pubescens, holarrhena antidysenterica..
Subject : Toxicology
Article Type : Original article (research)
Article File : Full Text PDF
Abstract : The objective of the study is to screen ot various phytochemicals and to evaluation the antioxidative and hepatoprotective activity of leaves of holarrhena pubescens wall (holarrhena antidysenterica). Material and Method: The antioxidant activity was determined by DDPH (2, 2-diphenyl-1-1-picrylhydrazyl) radical scavenging activity where ascorbic acid was control. The antioxidant property was later exploited and the ethanolic extract of plant was tested for hepatoprotective activity in CCl4 induced albino rats. The extract was tested for its hepatoprotective activity at three doses 100, 200 and 300 mg/kg respectively where silymarin 25 mg/kg was taken as standard reference drug. All results are presented as mean+SD (Standard Deviation). Significant differences between experimental groups were determined by tukey’s test. Results: The ethanolic extract showed strong antioxidant activity with inhibition of more than 90% DDPH free radicals at concentration of 80μg/ml. The hepatoprotective activity of ethanolic extract on CCl4 induced albino rats test were significant (p≤0.05) for effect of 300 mg/kg after 24hrs of treatment and (p≤0.01) for 100 mg/kg of extract after half hour of treatment compared to control. Conclusion: The presence of flavonoids, phenolic compound suggested that they may be partially responsible for antioxidant and hepatoprotective activity.
Article by : ASHWIN SINGH CHOUHAN
Article add date : 2021-10-14
How to cite : Ashwin singh chouhan. (2021-October-14). Evaluation of antioxidative and hepatoprotective activity of leaves of holarrhena pubescens wall ex.g.don. retrieved from https://openacessjournal.com/abstract/866